Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin?

By M. Bairu, W. Stirk, K. Doležal, J. van Staden. Plant Cell Tissue and Organ Culture, 2007

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The role of meta-topolins in alleviating micropropagation problems (2011, background): “The choice of plant growth regulators has been reported to play a crucial role in solving or alleviating most of these problems (Werbrouck et al. 1995, 1996; Bairu et al. 2007, 2008, 2009).”
Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult- and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes (2009, background): “More recently, mT has been shown to be the preferred cytokinin over BA for shoot multiplication, enhanced rooting, reduced hyperhydricity, and enhanced acclimatization ex vitro of Aloe polyphylla (Bairu et al. 2007).”
Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.) (2011, result): “Bairu et al. (2007) reported that plants treated with mT showed faster growth and superior rooting compared to BA.”
Influence of gelling agent and cytokinins on the control of hyperhydricity in Aloe polyphylla (2010, methodology): “High regeneration and low hyperhydricity were achieved by using meta-topolin (Bairu et al. 2007), a combination of low concentrations of cytokinins and NH4NO3 (Ivanova and Van Staden 2008), using nitrate or glutamine as the sole nitrogen source (Ivanova and Van Staden 2009), decreasing the ratio of…”