Loop-mediated isothermal amplification of DNA.

By T. Notomi, H. Okayama, Harumi Otawara-shi Masubuchi, Toshihiro Yonekawa, Keiko Watanabe, N. Amino, T. Hase. Nucleic Acids Research, 2000

7,677 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/2060fe4b01ec2a5bdb51eb47e5c0b111150d0cc5

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Citations in the corpus

Detection (2020, neutral): “The loop-mediated isothermal amplification (LAMP) method, which uses strand displacement DNA synthesis by Bst DNA polymerase, and amplifies the targets without thermal cycler, was considered to be a promising POCT method.(7) However, the prominent shortage of LAMP is the frequent non-specific amplif”
In‐field molecular diagnosis of plant pathogens: recent trends and future perspectives (2018, background): “The final products of a LAMP reaction are stem-loop DNAs with several inverted repeats of the target and cauliflower-like structures, allowing the amplification of 10(9) copies of the target sequence in less than an hour (Notomi et al., 2000).”
Advances in rapid detection methods for foodborne pathogens. (2014, methodology): “Most recently, a novel nucleic acid amplification method known as LAMP has been demonstrated as a rapid, low-cost, easy operating, highly sensitive, and specific detection method applied in several fields [70].”
Exploiting generic platform technologies for the detection and identification of plant pathogens (2008, background): “As an example, probably the most advanced isothermal amplification technology is loop-mediated amplification, which uses a highly processive strand displacing polymerase to separate the DNA strands, and primers that form loops to generate new priming sites (Notomi et al. 2000).”
Diagnosing COVID-19: The Disease and Tools for Detection (2020, methodology): “61 The drawbacks to LAMP are the challenges of optimizing primers and reaction conditions.”
Development and validation of genetic markers for sex and cannabinoid chemotype in Cannabis sativa L. (2020, background): “PACE has the further advantage of lower cost while retaining simple codominance, unlike loop-mediated isothermal amplification (LAMP; Notomi et al., 2000) or direct sequencing (McKernan et al.”
REVIEW: The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology (2014, methodology): “Alternative methods of PCR such as loop-mediated isothermal amplification (Notomi et al. 2000) or recombinase polymerase amplification (Piepenburg et al.”
Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance (2012, background): “An optimized protocol for DNA extraction from wheat seeds and Loop-Mediated Isothermal Amplification (LAMP) to detect Fusarium graminearum contamination of wheat grain.”