Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice.

By S. Toki, N. Hara, K. Ono, H. Onodera, A. Tagiri, S. Oka, Hiroshi Tanaka. The Plant Journal, 2006

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Advances in Transgenic Rice Biotechnology (2007, background): “Recently, Toki et al. (2006) reported the successful regeneration of transgenic plants within a month after the start of the aseptic culture of mature seeds by using scutellum from one day precultured seeds as explants, thus avoiding the risk of somaclonal variations.”
The Hologenome Concept: Human, Animal and Plant Microbiota (2014, background): “The mode of infection by A. tumefaciens is interesting not only biologically but because it has provided the basis for genetic engineering of plants (Toki et al. 2006).”
Phosphate deficiency signaling pathway is a target of arsenate and phosphate transporter OsPT1 is involved in As accumulation in shoots of rice (2013, methodology): “Nipponbare) were transformed according to a previous report (Toki et al. 2006).”
Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation[OPEN] (2016, methodology): “However, to date, the closest researchers have come to directly using mature seed tissues for transformation in monocots has been a report inwhich abrief preculture period (1 to 5 d) was used before Agrobacterium-mediated DNA delivery in japonica rice (Toki et al., 2006).”