Cytological Effect of the Potassium Metabisulphite and Potassium Nitrate Food Preservative on Root Tips of Allium cepa L.
By Ayşe Nihal Gömürgen. 2005
97 citations recorded by the source corpus.
The effects of the food preservatives potassium metabisulphite (PMB) and potassium nitrate (PN) have been studied on root tips of Allium cepa L. (variety Kantartopu-3). Roots of A.cepa were treated with a series of concentrations, ranging from 50 ppm to 100,000 ppm for 3, 6, 12, 24 and 48 h. Examinations of roots were done in permanent root tip squash preparations stained by the Feulgen technique. PMB and PN effect on the relative duration of each mitotic stage as compared with the control. They are also caused reduction in the mitotic index, indicating mitotic inhibition and increased frequency of abnormal mitosis.The type of abnormalities induced are chromosome stickiness, c-metaphase, anaphase and telophase bridges, disturbed chromosomes of anaphase and telophase stages, anaphase lagging and forward chromosomes at anaphase and telophase and micronuclei formation at intephase cells.
Subfields: Analytical Chemistry, Food Science, Plant Science
View Paper: https://www.semanticscholar.org/paper/1e863e13bb8856ddfd69c7484b3472461e1f986e
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In Analytical Chemistry
In Food Science
In Plant Science
Citations in the corpus
- Allium Cepa Root Chromosomal Aberration Assay: A Review (2013, unknown): “The presence of binucleated cells was reported by several investigators in several genera following chemical treatments [67].”
- Determination of genotoxic effects of chlorfenvinphos and fenbuconazole in Allium cepa root cells by mitotic activity, chromosome aberration, DNA content, and comet assay (2012, background): “gen and potential carcinogen, as previously demonstrated on human lymphocytes and root tips imply that it causes several chemicals damage on DNA [37–39].”