Influence of gelling agents on culture medium gel strength, water availability, tissue water potential, and maturation response in embryogenic cultures of Pinus strobus L.

By K. Klimaszewska, M. Bernier-Cardou, D. Cyr, B. Sutton. In vitro cellular & developmental biology. Plant, 2000

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Maturation of somatic embryos in conifers: Morphogenesis, physiology, biochemistry, and molecular biology (2002, background): “Higher concentrations of gelling agents stimulated pine somatic embryos by reducing water availability (Klimaszewska and Smith, 1997; Klimaszewska et al., 2000).”
Recent Progress in Somatic Embryogenesis of Four Pinus spp. (2007, methodology): “…and improving SE initiation frequencies in a large number of seed families, and subsequently, to the development of protocols for somatic embryo maturation and production of plants (Klimaszewska and Smith 1997; Garin et al. 1998; Klimaszewska et al. 2000, 2001, 2004; Park et al. 2006) (Fig.”
Forest biotechnology: Innovative methods, emerging opportunities (2005, background): “%) of Gelrite (Klimaszewska et al., 2000).”
Optimized somatic embryogenesis in Pinus strobus L. (2001, methodology): “Bulking and somatic embryo maturation protocols were the same as previously described (Klimaszewska and Smith, 1997; Klimaszewska et al., 2000), but with some modifications.”