454 Pyrosequencing analyses of forest soils reveal an unexpectedly high fungal diversity.

By M. Buée, M. Reich, C. Murat, E. Morin, R. Henrik Nilsson, S. Uroz, Francis Martin. New Phytologist, 2009

981 citations recorded by the source corpus.

Subfields: Cell Biology, Ecology, Plant Science

View Paper: https://www.semanticscholar.org/paper/1d847d795ed5bc2941e2f023275f7fa7844834ef

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Citations in the corpus

Variation in forest soil fungal diversity along a latitudinal gradient (2013, result): “The significant correlation between the diversity of the greater fungal community and mycorrhizal fungi is consistent with the work of Bueé et al. (2009) and Voříšková and Baldrian (2013), who showed that mycorrhizal fungi were prevalent in all the fungal communities sampled in their study.”
Colletotrichum – current status and future directions (2012, background): “This does not mean that data from single loci such as ITS do not have wide application, for example in environmental sequencing ( e.g. Buée et al .”
Plant intraspecific variation modulates nutrient cycling through its below ground rhizospheric microbiome (2019, methodology): “On ECM root tip DNA, the internal transcribed spacer region ITS‐1 was amplified with the primer pair ITS1F‐ITS2 adapted for pyrosequencing (Buée et al., 2009).”
The Potential of Metagenomic Approaches for Understanding Soil Microbial Processes (2014, methodology): “The initial application of this high-throughput sequencing technology explored the taxonomic diversity and composition of soil microbial communities using a polymerase chain reaction (PCR)-based approach that focused on phylogenetically informative ribosomal genes (Buée et al., 2009; Roesch et al., ”
High-Coverage ITS Primers for the DNA-Based Identification of Ascomycetes and Basidiomycetes in Environmental Samples (2012, background): “For example, since the average read length obtained from 454 pyrosequencing has until recently been 250 bp, pioneering studies on fungal metagenomics usually target the ITS1 region for DNA barcoding [9,46,51,52].”
Illumina metabarcoding of a soil fungal community (2013, background): “Even if the full ITS is amplified, the most common 454 chemistry recovers either ITS1 or ITS2 in full length, while the sequenced fraction of the other ITS subfragment is too short for inferences (Bálint et al., 2013; Bazzicalupo et al., 2013).”
Next‐generation sequencing technologies for environmental DNA research (2012, background): “Other studies have focused on soil fungal diversity in both forest and agricultural settings by analysing ITS amplicons (Acosta-Martı́nez et al. 2008; Buée et al. 2009; Jumpponen et al. 2010; Rousk et al. 2010).”