Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19
By A. Silvin, Nicolas Chapuis, G. Dunsmore, A. Goubet, A. Dubuisson, Lisa Derosa, Carole Almire, C. Hénon, Olivier Kosmider, N. Droin and 35 more. Cell, 2020
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Blood myeloid cells are known to be dysregulated in coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2. It is unknown whether the innate myeloid response differs with disease severity and whether markers of innate immunity discriminate high-risk patients. Thus, we performed high-dimensional flow cytometry and single-cell RNA sequencing of COVID-19 patient peripheral blood cells and detected disappearance of non-classical CD14LowCD16High monocytes, accumulation of HLA-DRLow classical monocytes (Human Leukocyte Antigen - DR isotype), and release of massive amounts of calprotectin (S100A8/S100A9) in severe cases. Immature CD10LowCD101−CXCR4+/− neutrophils with an immunosuppressive profile accumulated in the blood and lungs, suggesting emergency myelopoiesis. Finally, we show that calprotectin plasma level and a routine flow cytometry assay detecting decreased frequencies of non-classical monocytes could discriminate patients who develop a severe form of COVID-19, suggesting a predictive value that deserves prospective evaluation.
Subfields: Immunology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/1cd1a49ea236d722636c97815c8db88f1a648fce
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Citations in the corpus
- COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas (2021, positive): “Multiple studies have suggested the alterations of immune responses as one of the key mechanisms for severe symptoms (Guo et al., 2020; Schulte-Schrepping et al., 2020; Silvin et al., 2020; Wen et al., 2020; Zhang et al., 2020a, 2020b).”
- Synergism of TNF-α and IFN-γ Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARS-CoV-2 Infection and Cytokine Shock Syndromes (2020, neutral): “(Silvin et al., 2020) (ArrayExpress: E-MTAB-9221), Hadjadj et al.”