iPSC-derived human microglia-like cells to study neurological diseases
By E. Abud, Ricardo N. Ramirez, E. Martinez, Luke M. Healy, Cecilia H. H. Nguyen, Sean A. Newman, Andriy V. Yeromin, Vanessa M. Scarfone, S. Marsh, Cristhian Fimbres and 13 more. Neuron, 2017
989 citations recorded by the source corpus.
Summary Microglia play critical roles in brain development, homeostasis, and neurological disorders. Here, we report that human microglial-like cells (iMGL) can be differentiated from iPSCs to study their function in neurological diseases, like Alzheimer’s disease (AD). We find that iMGLs develop in vitro similarly to microglia in vivo and whole transcriptome analysis demonstrates that they are highly similar to cultured adult and fetal human microglia. Functional assessment of iMGLs reveals that they secrete cytokines in response to inflammatory stimuli, migrate and undergo calcium transients, and robustly phagocytose CNS substrates. iMGLs were used to examine the effects of Aβ fibrils and brain-derived tau oligomers on AD-related gene expression and to interrogate mechanisms involved in synaptic pruning. Furthermore, iMGLs transplanted into transgenic mice and human brain organoids resemble microglia in vivo. Together, these findings demonstrate that iMGLs can be used to study microglial function, providing important new insight into human neurological disease.
Subfields: Cellular and Molecular Neuroscience, Developmental Neuroscience, Neurology
View Paper: https://www.semanticscholar.org/paper/1c55b1a84a53da83604c775a779bac404d9514ee
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In Cellular and Molecular Neuroscience
In Developmental Neuroscience
In Neurology
Citations in the corpus
- Brain organoids: advances, applications and challenges (2019, background): “In these structures, interneurons generated from the ventral domain preferentially migrate towards the dorsal domain, resembling the tangential migration of interneurons from the subpallium to the cerebral cortex in vivo (Anderson et al., 1997).”