Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.

By K. Mullis, F. Faloona, S. Scharf, R. Saiki, G. Horn, H. Erlich. 1986

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Citations in the corpus

Atmospheric Movement of Microorganisms in Clouds of Desert Dust and Implications for Human Health (2007, methodology): “Nucleic acid amplification methods target regions of conserved genes, such as those found in the ribosomal genes, for use in identification (42, 167).”
Mass spectrometry tools for the classification and identification of bacteria (2010, background): “In principle, low levels of bacteria can be detected quantitatively in complex samples, owing to the efficient and specific amplification of targeted regions of microbial DNA by PC”
Viruses infecting bivalve molluscs (2004, unknown): “Procedures to detect herpesvirus in French oysters using the polymerase chain reaction (PCR) (Saiki et al. 1985; Mullis et al. 1986) were developed (Renault et al. 2000a; Arzul et al. 2001a,b,c).”
When Risks Cannot Be Seen: Regulating Uncertainty in Emerging Technologies (2017, unknown): “In contrast, the number of constituents in genetic engineering is high, due to the need for accurate positioning of nucleotides in extensive DNA strings (Mullis et al., 1986).”
Pathogen detection: a perspective of traditional methods and biosensors. (2007, background): “It was develped in the mid 80s (Mullis et al., 1986) and it is very widely sed in bacterial detection.”
DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing (2018, methodology): “The PCR technique was developed by Cary Mullis in 1983, as a technique which could amplify a small quantity of DNA without the application of any living organisms [13].”
Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of Arthropods (2007, methodology): “The field of biological diagnostics was revolutionized with the advent of the polymerase chain reaction (PCR) in the mid-1980s (Saiki et al. 1985, 1988; Mullis et al. 1986).”
A high-density linkage map of Theobroma cacao L. (2000, background): “They are highly polymorphic and can be amplified by PCR ( Mullis et al. 1986 ) with unique flanking primers (Beckman and Soller 1990).”
Assessment of the Genetic Diversity in Forest Tree Populations Using Molecular Markers (2014, background): “Finally, the possibility to rapidly amplify specific DNA fragments in vitro via polymerase chain reaction (PCR) [23] revolutionized the generation of molecular markers, leading to diverse sets of diagnostic DNA-marker systems with or without a priori sequence knowledge, such as random amplified poly”
Monitoring species of arbuscular mycorrhizal fungi in planta and in soil by nested PCR: application to the study of the impact of sewage sludge (2000, methodology): “Molecular techniques using PCR (Mullis et al., 1986; Saiki et al., 1988), PCR associated with systematic sequencing or RFLP (Di Bonito et al.”
Genetic Variation and Molecular Biogeography of a North American Invasive Plant Species (Alliaria petiolata, Brassicaceae) (2001, methodology): “Samples were amplified by the PCR (Mullis et al. 1986) in a 29-mL volume containing 20 mL water, 2.5 mL 10# Taq PCR buffer (Gibco BRL), 2 mL MgCl2 (50 mM), 2 mL dNTP mix (10 mM), 0.5 mL bovine serum albumin (4 mg/mL), 0.25 mL primer (20 mM), 0.25 mL recombinant Taq DNA polymerase (Gibco BRL), and 1.”
Substantial biases in ultra-short read data sets from high-throughput DNA sequencing (2008, methodology): “The adoption of high-throughput sequencing will revolutionize molecular biology research, similar to the invention of the polymerase chain reaction (PCR) twenty years ago (3).”
The role of molecular genetics in sculpting the future of integrative biogeography (2008, background): “…et al., 1987; Riddle and Honeycutt, 1990; Bernatchez, 1991; Lovette et al., 1999; Kuchta, 2007), The advent of the polymerase chain reaction (PCR, Mullis et al., 1986), coupled with the development of chain-terminating DNA sequencing (Sanger et al., 1977), lead to the ability to sequence DNA…”