Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays

By Meghana Kulkarni, M. Booker, S. Silver, Adam A. Friedman, Pengyu Hong, Norbert Perrimon, B. Mathey-Prevot. 2006

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Causal inference by using invariant prediction: identification and confidence intervals (2015, methodology): “As an example we mention gene knock-out experiments with oﬀ-target eﬀects in biology [e.g. Jackson et al., 2003, Kulkarni et al., 2006].”
CellProfiler Analyst: data exploration and analysis software for complex image-based screens (2008, result): “Our work therefore confirms, with non-overlapping dsRNAs, a recently reported cell cycle regulation role for widerborst [36] and together indicates that it is unlikely this phenotype is due to off-target effects [37,38].”
Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes. (2008, unknown): “edu/) or S2 cell cDNA library (Kulkarni et al. 2006).”
RNAi-Based Insecticidal Crops: Potential Effects on Nontarget Species (2013, background): “Forum www.biosciencemag.org August 2013 / Vol. 63 No. 8 • BioScience 659 Forum that 17% of siRNAs had complete sequence homologies with off-target binding sites in the Drosophila melanogaster genome (Kulkarni et al. 2006).”