Circular RNA ITCH has inhibitory effect on ESCC by suppressing the Wnt/β-catenin pathway

By Fang Li, Liyuan Zhang, Wei Li, Jieqiong Deng, Jian Zheng, Mingxing An, Jiachun Lu, Yifeng Zhou. OncoTarget, 2015

661 citations recorded by the source corpus.

Circular RNAs with exonic sequences represent a special form of non-coding RNAs, discovered by analyzing a handful of transcribed genes. It has been observed that circular RNAs function as microRNA sponges. In the present study, we investigated whether the expression of circular RNAs is altered during the development of esophageal squamous cell carcinoma (ESCC). Using a TaqMan-based reverse transcriptase polymerase chain reaction assay, the relationship between cir-ITCH and ESCC was analyzed in a total of 684 ESCC and paired adjacent non-tumor tissue samples from eastern and southern China. We found that cir-ITCH expression was usually low in ESCC compared to the peritumoral tissue. The functional relevance of cir-ITCH was further examined by biochemical assays. As sponge of miR-7, miR-17, and miR-214, cir-ITCH might increase the level of ITCH. ITCH hyper expression promotes ubiquitination and degradation of phosphorylated Dvl2, thereby inhibiting the Wnt/β-catenin pathway. These results indicate that cir-ITCH may have an inhibitory effect on ESCC by regulating the Wnt pathway.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/1a5fcac47111b4a068958888f4e5d82bbb090339

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In Molecular Biology

1. M. Gorospe
2. W. Du
3. Minghua Wu
4. Yvonne Tay
5. J. Martindale
6. Weining Yang
7. Zhenguo Yang
8. J. J. Chan

Citations in the corpus

• Identification of HuR target circular RNAs uncovers suppression of PABPN1 translation by CircPABPN1 (2017, background): “For example, the circRNA CiRS-7 contains multiple sites for miR-7, enabling it to sequester miR-7 and thereby decreasing its availability to target mRNAs bearing miR-7 sites; likewise, circSRY sponges miR-138, and circITCH sponges miR-7, miR-17, and miR-214.(10,12,14,15) These examples indicate that”
• Noncoding RNA:RNA Regulatory Networks in Cancer (2018, background): “Not surprisingly, circ-ITCH has been reported to antagonize miR-7, miR-17 and miR-214, to upregulate ITCH and impede lung and esophageal squamous cell carcinoma growth by blocking the Wnt/β-catenin pathway [156,157].”
• CircRNA: functions and properties of a novel potential biomarker for cancer (2017, background): “Studies have proven that the circular RNAs CDR1as or ciRS-7 [11, 12], circ-SRY [12], circ-ITCH [8], circ- HIPK3 [13], hsa_circ_0000615 [14], and mm9_circ_012559 [15] are able to act as miRNA sponges.”
• CircRNA: a novel type of biomarker for cancer (2017, background): “With the progress of exploring circRNAs, it is found that circRNA-ITCH is the sponge for miR-7, miR-17, and miR-214 [20].”
• Foxo3 circular RNA retards cell cycle progression via forming ternary complexes with p21 and CDK2 (2016, background): “Due to their abundance and stability, circular RNAs are believed to be more effective relative to noncircular RNAs in sponging miRNA (1,7,9).”