Flexibility in the ABC transporter MsbA: Alternating access with a twist

By A. Ward, C. Reyes, Jodie Yu, Christopher B. Roth, G. Chang. Proceedings of the National Academy of Sciences of the United States of America, 2007

768 citations recorded by the source corpus.

Subfields: Molecular Medicine, Nutrition and Dietetics, Oncology

View Paper: https://www.semanticscholar.org/paper/19c88fd36bef9a06b6ffd36ba8f8df3f93d2f547

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Medicine

1. H. Nikaido
2. Xian-Zhi Li
3. E. Dassa
4. C. Orelle
5. K. Locher
6. A. Davidson
7. Jue Chen
8. Yigong Shi

In Nutrition and Dietetics

1. E. Dassa
2. C. Orelle
3. Xian-Zhi Li
4. H. Nikaido
5. A. Davidson
6. Jue Chen
7. Yigong Shi

In Oncology

1. Xian-Zhi Li
2. H. Nikaido
3. K. Locher
4. E. Dassa
5. C. Orelle
6. A. Davidson
7. Jue Chen
8. S. Wilkens

Citations in the corpus

• Efflux-Mediated Drug Resistance in Bacteria: an Update (2009, background): “coli lipid flippase) further supports that the inward and outward openings are mediated by two different sets of transmembrane helix interactions and that large ranges of motion may be required for substrate transport.([103]) Functional expression of Sav1866 in L.”
• Handbook of ion channels (2015, background): “The twofold symmetry of MSDs seen in other ABC exporters (Dawson and Locher, 2006; Ward et al., 2007; Aller et al., 2009) (Figure 42.”
• A glimpse of structural biology through X-ray crystallography. (2014, background): “Crystal structures, including a bacterial multidrug transporter Sav1866 (Dawson and Locher, 2006), a putative metal-chelate-type transporter (Pinkett et al., 2007), a maltose transporter (Oldham et al., 2007), BtuCD bound to the periplasmic-binding protein BtuF (Hvorup et al., 2007), a bacterial lip”
• Mechanistic diversity in ATP-binding cassette (ABC) transporters (2016, background): “There is currently only one structurally defined fold of ABC exporters, first revealed by the bacterial Sav1866 protein 54 and subsequently found in all reported crystal structures of ABC exporters 34,55,57,58,63,66,91-96 .”
• Structure and mechanism of ABC transporters (2015, background): “Ward A, Reyes CL, Yu J, Roth CB, Chang G: Flexibility in the ABC transporter MsbA: Alternating access with a twist.”
• Structure, Function, and Evolution of Bacterial ATP-Binding Cassette Systems (2008, background): “New structures have now been released (518), but a significant amount of work published since 2001 that was based on the retracted structures will require reevaluation.”
• 3D structures of membrane proteins from genomic sequencing (2012, positive): “…the ABC transporter S. typhimurium MsbA, evolutionary couplings between trans-membrane helix 2 and transmembrane helices 5 and 6 are false positives with respect to monomer structure but true positives with respect to the crystal structure dimer interface (PDB: 3b60; Ward et al., 2007) (Figure 4A).”
• Overcoming ABC transporter-mediated multidrug resistance: Molecular mechanisms and novel therapeutic drug strategies. (2016, background): “…spectroscopy studies indicated that MsbA can form a large and flexible chamber facing the cytoplasmic Page 8 of 56 Ac ce pt ed M an us cr ip t 8 region to allow for the flipping of large molecules such as lipid A or lipopolysaccharide (LPS) from the cytoplasm to the exterior (Ward et al., 2007).”