Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins

By J. Nam, C. S. Thaxtin, Chad A. Mirkin, Becky Kusko. Science, 2003

2,268 citations recorded by the source corpus.

An ultrasensitive method for detecting protein analytes has been developed. The system relies on magnetic microparticle probes with antibodies that specifically bind a target of interest [prostate-specific antigen (PSA) in this case] and nanoparticle probes that are encoded with DNA that is unique to the protein target of interest and antibodies that can sandwich the target captured by the microparticle probes. Magnetic separation of the complexed probes and target followed by dehybridization of the oligonucleotides on the nanoparticle probe surface allows the determination of the presence of the target protein by identifying the oligonucleotide sequence released from the nanoparticle probe. Because the nanoparticle probe carries with it a large number of oligonucleotides per protein binding event, there is substantial amplification and PSA can be detected at 30 attomolar concentration. Alternatively, a polymerase chain reaction on the oligonucleotide bar codes can boost the sensitivity to 3 attomolar. Comparable clinically accepted conventional assays for detecting the same target have sensitivity limits of ∼3 picomdar, six orders of magnitude less sensitive than what is observed with this method.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/19b5f19e08dd4bde1f6561c077be033ca40114d6

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In Biomedical Engineering

In Molecular Biology

Citations in the corpus

Noble Metal Nanoparticles for Biosensing Applications (2012, background): “specific targets and gold nanoprobes that harbored a secondary recognition agent and hundreds of ssDNA barcodes strands [61,62].”