Effective targeted gene ‘knockdown’ in zebrafish
By Aidas Nasevicius, S. Ekker. Nature Genetics, 2000
2,653 citations recorded by the source corpus.
Subfields: Cell Biology, Molecular Biology, Pediatrics, Perinatology and Child Health
View Paper: https://www.semanticscholar.org/paper/19388e0c0d8203b409ec307330bf3ec8c6fbf14d
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In Cell Biology
- C. Lawrence
- D. Stainier
- Francesca Peri
- Christiane Nüsslein-Volhard
- Jarema Malicki
- Xiangyun Wei
- Carl Smith
- R. Spence
In Molecular Biology
- D. Stainier
- Francesca Peri
- Christiane Nüsslein-Volhard
- Jarema Malicki
- Xiangyun Wei
- C. Lawrence
- J. Eisen
- Nathan D. Lawson
In Pediatrics, Perinatology and Child Health
- Christiane Nüsslein-Volhard
- Jarema Malicki
- Xiangyun Wei
- Francesca Peri
- D. Stainier
- J. Heasman
- J. Campos-Ortega
- Benedikt Geldmacher-Voss
Citations in the corpus
- Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut (2005, methodology): “To knock down gene expression in all cells, MO were injected between the one- and four-cell stages as previously described (Nasevicius and Ekker, 2000).”
- Cellular and molecular analyses of vascular tube and lumen formation in zebrafish (2005, methodology): “5199”
- Characterization of NF-κΒ/IκΒ Proteins in Zebra Fish and Their Involvement in Notochord Development (2004, methodology): “To better correlate the observed mIBM phenotype with blockage of NF- activity in vivo, we designed morpholinos ( 26 ) for two zebra fish NF- genes, p100 and p65, and performed single- and double-knockdown experiments, with 2.3 to 4.5 ng of each morpholino.”
- Roles for GFRα1 receptors in zebrafish enteric nervous system development (2004, positive): “To determine whether gfra1aand gfra1bare necessary for enteric nervous system development, we used morpholino antisense oligonucleotides to block their function (Nasevicius and Ekker, 2000).”
- A 90° rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells (2003, methodology): “A morpholino-modified antisense ASIP oligonucleotide (Nasevicius and Ekker, 2000), designed according to the manufacturer’s recommendations, was obtained from Gene Tools, LLC.”
- The ankyrin repeat protein Diversin recruits Casein kinase Iε to the β-catenin degradation complex and acts in both canonical Wnt and Wnt/JNK signaling (2002, background): “4 ng of mRNA (TL strain) or 1–6 ng of antisense morpholinos (AB strain; Hammerschmidt et al. 1996; Nasevicius and Ekker 2000).”
- Control of chemokine-guided cell migration by ligand sequestration. (2008, methodology): “To determine whether CXCR7 plays a role in the migration of PGCs, we have knocked down its activity using morpholino an- 464 Cell 132, 463–473, February 8, 2008 ª2008 Elsevier Inc. tisense oligonucleotides (Nasevicius and Ekker, 2000) (Figure S1).”
- The behaviour and ecology of the zebrafish, Danio rerio (2007, background): “(1) Taxonomic status The zebrafish belongs to the family of freshwater fishes Cyprinidae, the most species-rich vertebrate family (Nelson, 1994).”
- Medaka — a model organism from the far east (2002, background): “The expression patterns of genes or ESTs that map to these regions can then be used to identify candidate genes, which can eventually be analysed in rescue or morpholino knock-down experiment”