Empirically-controlled mapping of the Caenorhabditis elegans protein-protein interactome network

By Nicolas Simonis, Jean‐François Rual, Anne-Ruxandra Carvunis, Murat Tasan, I. Lemmens, T. Hirozane-Kishikawa, Tong Hao, Julie M. Sahalie, K. Venkatesan, Fana Gebreab and 29 more. Nature Methods, 2009

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To provide accurate biological hypotheses and elucidate global properties of cellular networks, systematic identification of protein-protein interactions must meet high quality standards. We present an expanded C. elegans protein-protein interaction network, or 'interactome' map, derived from testing a matrix of ∼10,000 × ∼10,000 proteins using a highly specific, high-throughput yeast two-hybrid system. Through a new empirical quality control framework, we show that the resulting data set (Worm Interactome 2007, or WI-2007) was similar in quality to low-throughput data curated from the literature. We filtered previous interaction data sets and integrated them with WI-2007 to generate a high-confidence consolidated map (Worm Interactome version 8, or WI8). This work allowed us to estimate the size of the worm interactome at ∼116,000 interactions. Comparison with other types of functional genomic data shows the complementarity of distinct experimental approaches in predicting different functional relationships between genes or proteins.

Subfields: Aging, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/18f7aa61039c2eedaeceb5980be640d23bb211ae

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