Scanometric DNA array detection with nanoparticle probes.
By T. Taton, Chad A. Mirkin, R. Letsinger. Science, 2000
2,051 citations recorded by the source corpus.
A method for analyzing combinatorial DNA arrays using oligonucleotide-modified gold nanoparticle probes and a conventional flatbed scanner is described here. Labeling oligonucleotide targets with nanoparticle rather than fluorophore probes substantially alters the melting profiles of the targets from an array substrate. This difference permits the discrimination of an oligonucleotide sequence from targets with single nucleotide mismatches with a selectivity that is over three times that observed for fluorophore-labeled targets. In addition, when coupled with a signal amplification method based on nanoparticle-promoted reduction of silver(I), the sensitivity of this scanometric array detection system exceeds that of the analogous fluorophore system by two orders of magnitude.
Subfields: Biomedical Engineering, Electronic, Optical and Magnetic Materials, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/18a1ec9535ebf1444bdb93ce796fd065c0cc3103
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In Biomedical Engineering
- George M. Whitesides
- Lee Josephson
- C. Sönnichsen
- Donald E. Ingber
- Shengshui Hu
- Fang Wang
- D. Giljohann
- Chad A. Mirkin
In Electronic, Optical and Magnetic Materials
In Molecular Biology
Citations in the corpus
- Functionalized Gold Nanoparticles for Applications in Bionanotechnology (2005, background): “…Simard et al. 2000), phosphines (Franzen et al. 2002), oligonucleotides/DNA (Storhoff et al. 1998, 1999, 2000; Wang et al. 2002; Park et al. 2002; Taton et al. 2000; Demers et al. 2000; Elghanian et al. 1997; Mirkin 2000a), amines (Gomez et al. 2000), proteins (Hayat 1989; Tkachenko et al. 2003;…”
- Soft lithography in biology and biochemistry. (2001, background): “A common strategy in microarray or microfluidic devices is to utilize external, separate, macroscopic detectors, such as slide readers, epi-fluorescence optical microscopes, and confocal microscopes (150, 183).”