Measuring bacterial biomass production and growth rates from leucine incorporation in natural aquatic environments

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Diversity, abundance, and biomass production of bacterial groups in the western Arctic Ocean (2007, methodology): “46 Malmstrom et al.: Diversity and activity of Arctic bacterioplankton Bacterial biomass production was estimated from total leucine incorporation rates (Kirchman 2001).”
Does nutrient enrichment decouple algal–bacterial production in periphyton? (2008, methodology): “BBP was calculated from leucine uptake (nmol leucine/h) by assuming that the fraction of leucine in protein ¼ 7.3%, cellular C/protein ¼ 86%, and isotope dilution was negligible (Kirchman 2001).”
Viral control of bacterial growth efficiency in marine pelagic environments (2009, methodology): “…(see below) were used to estimate BR (O2 consumption rate was converted to C flux by assuming that the respiratory quotient 5 1; del Giorgio and Cole 1998), BP (leucine incorporation was converted to C flux by assuming a conversion factor of 125 mol C per mol leucine; Kirchman 2001), and VS.”
Changes in bacterial activity and community structure in response to dissolved organic matter in the Hudson River, New York (2004, methodology): “Bacterial growth was assayed at 5 time points (0, 24, 48, 54 and, 72 h) as the rate of incorporation of 3H-leucine (Smith & Azam 1992, Kirchman 2001).”