Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor

By Fen-Fen Zhang, Xiaoli Wang, S. Ai, Zhengdong Sun, Qiao Wan, Ziqiang Zhu, Y. Xian, Litong Jin, Katsunobu Yamamoto. 2004

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An amperometric glucose biosensor based on the immobilization of glucose oxidase on the ZnO nanotubes (2009, methodology): “In addition, a uric acid biosensor based on ZnO nanorods [ 20 ] and a cholesterol biosensor by immobilization of cholesterol oxidase onto ZnO nanoporous thin film [21] have also been reported.”
Nanostructured Zinc Oxide Particles in Chemically Modified Electrodes for Biosensor Applications (2008, background): “5 which makes it suitable for absorption of proteins with low IEPs where the protein immobilization is primarily driven by electrostatic interaction (Zhang et al. 2004; Liu et al. 2005).”
Chemical sensors based on nanostructured materials (2007, background): “[39] reported ZnO nanorods for a reagentless ric acid biosensor.”
Potential applications of enzymes immobilized on/in nano materials: A review. (2012, background): “After 60 days of storage and intermittentlymeasuring every 5 days, the results showed that the response of the sensor still maintain about 80% of the original values (Zhang et al., 2004a, b).”
Selective potentiometric determination of uric acid with uricase immobilized on ZnO nanowires (2011, background): “These are the reasons why nanowires are more sensitive, have an extended detection limit and possess a fast response time as compared to a thin films [24-35].”