Getting to the fat of the matter: models, methods and assumptions for dealing with lipids in stable isotope analyses

By D. Post, C. Layman, D. Arrington, G. Takimoto, J. Quattrochi, Carman G. Montaña. Oecologia, 2007

2,464 citations recorded by the source corpus.

Subfields: Atmospheric Science, Ecology, Geochemistry and Petrology

View Paper: https://www.semanticscholar.org/paper/160c8263417439b510e1c8e8086b6c27b98f9864

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Citations in the corpus

The diet composition of immature loggerheads: Insights on trophic niche, growth rates, and fisheries interactions (2009, methodology): “We did not extract lipids from any tissue samples, but instead mathematically corrected for lipid content of samples following the methodology of Post et al. (2007).”
Widespread intraspecific organismal stoichiometry among populations of the Trinidadian guppy (2012, background): “Because these were field collections from wild populations, it was not possible to separate the environmental and genetic components of life-history phenotype.”
On the Use of Stable Isotopes in Trophic Ecology (2011, background): “Despite several recent reviews (Kelly 2000, Logan et al. 2008, Post et al. 2007, Mintenbeck et al. 2008), no consensus has emerged regarding the use and efficacy of lipid extraction.”
Diet and resource use among Greenland sharks (Somniosus microcephalus) and teleosts sampled in Icelandic waters, using δ13C, δ15N, and mercury (2010, methodology): “N ratios (C/N) of <3.2, indicating adequate lipid removal (C/N of <3.5 indicates adequate removal of lipid from animal tissues; Post et al. 2007).”
Ten years of experimental animal isotopic ecology (2009, positive): “Thus, some of the variation in Δ(13)C values is explained by a tissue’s lipid content (Post et al. 2007).”
Food Web Structure of the Alaskan Nearshore Shelf and Estuarine Lagoons of the Beaufort Sea (2012, methodology): “The lack of correlation between δ(13)C values and C/N ratios suggests that few if any of the organisms studied have carbon isotope composition affected by high lipid content; we therefore did not attempt to correct measured carbon isotope composition based upon C/N ratios (Post et al. 2007).”
The relative importance of mangroves and seagrass beds as feeding areas for resident and transient fishes among different mangrove habitats in Florida and Belize: Evidence from dietary and stable-isotope analyses (2012, methodology): “Variation in lipid content in an organism can affect δ(13)C values and thus ecological interpretations (Post et al., 2007).”
Characterizing dietary variability and trophic positions of coastal calanoid copepods: insight from stable isotopes and fatty acids (2009, methodology): “To remove the bias associated with heterogeneous lipid content, 22 samples of copepods with a range of C/N ratios from 4 to 16 were extracted to remove lipids prior to stable isotope analysis, following Post et al. (2007) .”
Trophic ecology of Pacific salmon (Oncorhynchus spp.) in the ocean: a synthesis of stable isotope research (2009, background): “Lipid content can alter d13C values if the C:N ratio is greater than 3.5 (Post et al. 2007).”
Stable isotope analysis reveals community-level variation in fish trophodynamics across a fringing coral reef (2012, background): “The average C/N ratio in the present study (4.01, Table 1) and the correction proposed by Post et al. (2007) suggest that the change in d 13 C due to lipid extraction (0.65”
Diet of young Atlantic bluefin tuna (Thunnus thynnus) in eastern and western Atlantic foraging grounds (2010, background): “In fish tissue, ratios of total carbon to nitrogen (C:N) are linearly related to lipid content (Post et al. 2007) and can be used to track changes in body condition (Estrada et al. 2005).”