Functional classification and experimental dissection of long noncoding RNAs
By F. Kopp, Joshua T. Mendell. Cell, 2018
2,967 citations recorded by the source corpus.
Over the last decade, it has been increasingly demonstrated that the genomes of many species are pervasively transcribed, resulting in the production of numerous long noncoding RNAs (lncRNAs). At the same time, it is now appreciated that many types of DNA regulatory elements, such as enhancers and promoters, regularly initiate bidirectional transcription. Thus, discerning functional noncoding transcripts from a vast transcriptome is a paramount priority, and challenge, for the lncRNA field. In this review, we aim to provide a conceptual and experimental framework for classifying and elucidating lncRNA function. We categorize lncRNA loci into those that regulate gene expression in cis versus those that perform functions in trans, and propose an experimental approach to dissect lncRNA activity based on these classifications. These strategies to further understand lncRNAs promise to reveal new and unanticipated biology, with great potential to advance our understanding of normal physiology and disease.
Subfields: Cancer Research, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/15d13c9d9253b3c9755c7cd21bcb89638d235c61
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In Cancer Research
In Molecular Biology
Citations in the corpus
- Mechanisms and Functions of Long Non-Coding RNAs at Multiple Regulatory Levels (2019, background): “Many animal lncRNA biological functions have been confirmed; however, the biological functions of only a few plant lncRNAs have been studied [4,5].”
- Noncoding RNA:RNA Regulatory Networks in Cancer (2018, background): “Several lncRNAs, such as XIST, H19, HOTAIR (Hox Transcript Antisense RNA), MALAT1 (Metastasis Associated Lung Adenocarcinoma Transcript 1), and NEAT1 (Nuclear Enriched Abundant Transcript 1), are well studied and known to play key regulatory roles in diverse processes, such as X inactivation, imprin”