Three-dimensional Tissue Culture Based on Magnetic Cell Levitation
By G. Souza, J. Molina, R. Raphael, M. Ozawa, Daniel J. Stark, C. Levin, L. Bronk, J. Ananta, J. Mandelin, M. Georgescu and 5 more. Nature Nanotechnology, 2010
619 citations recorded by the source corpus.
Cell culture is an essential tool for drug discovery, tissue engineering, and stem cell research. Conventional tissue culture produces two-dimensional (2D) cell growth with gene expression, signaling, and morphology that can differ from those in vivo and thus compromise clinical relevancy1–5. Here we report a three-dimensional (3D) culture of cells based on magnetic levitation in the presence of hydrogels containing gold and magnetic iron oxide (MIO) nanoparticles plus filamentous bacteriophage. This methodology allows for control of cell mass geometry and guided, multicellular clustering of different cell types in co-culture through spatial variance of the magnetic field. Moreover, magnetic levitation of human glioblastoma cells demonstrates similar protein expression profiles to those observed in human tumor xenografts. Taken together, these results suggest levitated 3D culture with magnetized phage-based hydrogels more closely recapitulates in vivo protein expression and allows for long-term multi-cellular studies.
View Paper: https://www.semanticscholar.org/paper/14b1e622f2f17dceec2c31fd8a79714ff8b84a56
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Citations in the corpus
- Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning (2018, methodology): “Magnetically levitated human glioblastoma cells closely recapitulated in vivo protein expression observed in human glioblastoma tumor xenografts (Souza et al., 2010) and recently, a high-throughput assay for toxicity screening in 3D cell cultures using magnetic levitation was described, making this…”