Fluorescence Lifetime Imaging (FLI) in Real-Time - a New Technique in Photosynthesis Research

By O. Holub, M. Seufferheld, C. Gohlke, Govindjee, Robert M. Clegg. Photosynthetica (Praha), 2000

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Chlorophyll fluorescence imaging of photosynthetic activity with the flash-lamp fluorescence imaging system (2005, background): “These techniques, however, only image the Chl fluorescence, i.e. one band in one wavelength region (e.g. Bro et al. 1995, Osmond et al. 1999, Holub et al. 2000, Nedbal et al. 2000, Baker et al. 2001, Chaerle and van der Straeten 2001, Barbagallo et al. 2003, Hill et al. 2004, Nedbal and Whitmarsh…”
CORAL PHOTOBIOLOGY STUDIED WITH A NEW IMAGING PULSE AMPLITUDE MODULATED FLUOROMETER 1 (2005, background): “The measuring principles applied range from relatively simple light modulation approaches (Siebke and Weis 1995a, Oxborough and Baker 1997, Grunwald and Kühl 2004) to advanced lifetime imaging systems (Holub et al. 2000).”
Non-Photochemical Quenching and Energy Dissipation in Plants, Algae and Cyanobacteria (2014, background): “[Note that change in fluorescence intensity can simply be due to change in the concentration of Chl; thus, measurement of lifetime of fluorescence is crucial in reaching firm conclusions; see, e.g., Holub et al. 2000, 2007].”