Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques

By M. Duren, R. Morpurgo, J. Doležel, R. Afza. Euphytica, 2004

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In vitro induction of tetraploid in pomegranate (Punica granatum) (2003, background): “In contrast to experiments with Zantedeschia (Cohen and Yao, 1996) and Musa (van In vitro comparison of roots and leaves between Duren et al., 1996), no dead shoots were observed. diploids and tetraploids showed that there was no Treated shoots recovered rapid growth after one- significant…”
Low-cost alternatives for the micropropagation of banana (2001, methodology): “In vitro shoot tips of ‘Grande Naine’, a commercially important dessert banana of the Cavendish subgroup, were bisected longitudinally and cultured in reusable Magenta boxes (Magenta Corporation, USA) containing 50 ml MS medium (Murashige and Skoog, 1962) with 20 µM BA, 40 mg l−1 cystein, 1 mg l−1 t”
Induction of tetraploid poplar and black locust plants using colchicine: chloroplast number as an early marker for selecting polyploids in vitro (2009, background): “Through tissue culture methods it became possible to treat large amounts of clonal shoot tips with colchicine, to regenerate new shoots and select tetraploids in vitro (Van Duren et al. 1996; Li et al. 2008).”