Electrophoretic mobility shift assay (EMSA) for detecting protein–nucleic acid interactions

By Lance M. Hellman, M. Fried. Nature Protocols, 2007

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The Post-GWAS Era: From Association to Function. (2018, background): “context.(88) Antibodies raised against candidate TFs can be added to the reaction to confirm TF binding to the variant; alternatively, purified recombinant protein can be used instead of nuclear extract to assess binding of specific TFs.”
Circular RNA-protein interactions: functions, mechanisms, and identification (2020, background): “EMSA is a common affinity electrophoresis technique used to study protein-DNA and protein-RNA interactions [110].”
Revealing protein–lncRNA interaction (2015, methodology): “The first proposed methods were low-throughput procedures able to identify only one or few RNAs linked to a protein: these include the RNA electrophoretic mobility shift assay [28], RNA pulldown assay [29], oligonucleotide-targeted RNase H protection assay [30] and FISH co-localization [31].”
Direct measurement of DNA affinity landscapes on a high-throughput sequencing instrument (2011, background): “1f ), possibly because dissociation within the gel reduces the dynamic range of the EMSA assa”