An optimized Agrobacterium-mediated transformation procedure for Phaseolus acutifolius A. Gray

By J. Clercq, M. Zambre, M. V. Montagu, W. Dillen, G. Angenon. Plant Cell Reports, 2002

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Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes (2006, methodology): “Both micro-particle bombardment (Gulati et al. 2002; Li et al. 2004) and A. 25 tumefaciens (De Clercq et al. 2002; Li et al. 2004) have been used for DNA delivery into 1 either embryogenic or organogenic cultures.”
Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus (2009, background): “Agrobacterium cells cultured overnight are usually in the late exponential phase of growth in which many cells have already reached the stationary phase (De Clercq et al. 2002).”
Beans (Phaseolus spp.) – model food legumes (2004, background): “…meantime the authors, as well as CIAT (see pp. 88– 89) have improved the protocol, so that three different varieties of P. acutifolius can now be transformed, and the efficiency is such that between 5–10 transformants can be obtained in one experiment (De Clercq et al., 2002; Zambre et al., 2003).”
Phaseolus Bean Improvement in Tanzania, 1959–2005 (2006, background): “Transformation is easier with tepary bean than with common bean ( De Clerq et al., 2002 ) and it should be possible to introduce genes from P. vulgaris into P. acutifolius by this method and then backcross into P. vulgaris (Broughton et al., 2003).”