Adaptation of a rapid and economical microcentrifugation method to measure thymidine and leucine incorporation by soil bacteria

By E. Bååth, M. Pettersson, Katarina H. Söderberg. 2001

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Plant genotype strongly modifies the structure and growth of maize rhizosphere microbial communities (2010, methodology): “Bacterial growth was estimated by the leucine incorporation technique (Bååth, 1994), as modified by Bååth et al. (2001).”
Soil microbial recolonisation after a fire in a Mediterranean forest (2011, methodology): “Centrifugation and washing were performed as described by Bååth et al. (2001).”
Revisiting the hypothesis that fungal-to-bacterial dominance characterizes turnover of soil organic matter and nutrients (2015, methodology): “…PLFA composition, and total respiration Bacterial growth was measured using the leucine (Leu) incorporation method (Kirchman et al. 1985) adapted for soil (Bååth et al. 2001) with modifications (Rousk et al. 2009), which estimates the rate of protein synthesis as a measure of bacterial growth.”
Adaptation of soil microbial communities to temperature: comparison of fungi and bacteria in a laboratory experiment (2009, methodology): “Bacterial growth was estimated using the leucine incorporation technique on bacteria extracted from soil using homogenization–centrifugation (Bååth, 1994; Bååth et al., 2001) with some modifications.”