AFM: a versatile tool in biophysics
By Andrea Alessandrini, Paolo Facci. 2005
367 citations recorded by the source corpus.
Here we review the applications of atomic force microscopy to the study of samples of biological origin. Emphasis is given to provide the reader with information on the broad range of different biophysical applications that, to date, such a technique can deal with. After recalling briefly the operating principles of an atomic force microscope, the broad field of bio-imaging applications is faced (DNA, DNA–protein interaction, proteins, lipid membranes, cells); thereafter, the use of the atomic force microscope to measure forces is introduced and force mapping on living cells is discussed. This section is followed by the description of the use of force curves in assessing single-molecule inter- and intramolecular interactions. A paragraph on the perspectives of the technique in biophysical applications concludes the paper. We hope that this review can help the reader in appreciating how atomic force microscopy contributes to the current explosive growth of nanobiosciences, where biology, chemistry and physics merge.
Subfields: Atomic and Molecular Physics, and Optics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/112b5a426a986afce1169e9636b266036b13fdb2
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Citations in the corpus
- Essential Oils in Food Preservation: Mode of Action, Synergies, and Interactions with Food Matrix Components (2012, background): “AFM has one important advantage over electron microscopy, in that it allows measurements in liquid under physiological conditions, avoiding difficult sample preparation and the artifacts associated herewith (Alessandrini and Facci, 2005).”
- Silicon Nanotweezers With Subnanometer Resolution for the Micromanipulation of Biomolecules (2008, background): “clude force-extension characterization, indentation, and combination of AFM with fluorescence microscopy [9].”