Biochemical analysis and optimization of inhibition and adsorption phenomena in glass–silicon PCR-chips
By Ivan Erill, S. Campoy, N. Erill, J. Barbé, J. Aguiló. 2003
105 citations recorded by the source corpus.
Abstract The use of glass–silicon chips for PCR analysis has been widely reported in the last decade, but there have been few systematic efforts to pin down the biochemical problems such systems bring forth. Here we report a systematic analysis of material-related inhibition and adsorption phenomena in glass–silicon PCR-chips. The results suggest that the previously reported inhibition of PCR by silicon-related materials stems mainly from the adsorption of Taq polymerase at chip walls due to increased surface-to-volume ratios, and not from a straight chemical action of silicon-related materials on the PCR-mix. In contrast to Taq polymerase, DNA is not adsorbed in noticeable amounts. The net effect of polymerase adsorption can be counteracted by the addition of a titrated amount of competing protein bovine serum albumin (BSA) and the ensuing reactions can be kinetically optimized in chips to yield effective amplifications in the whereabouts of 20 min.
Subfields: Biomedical Engineering, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/103d52cd0eade7ba88d644640b09f89d4117b573
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In Biomedical Engineering
Citations in the corpus
- PCR microfluidic devices for DNA amplification. (2006, methodology): “…microfluidics were anodically bonded to a glass cover to obtain a closed PCR system (Chaudhari et al., 1998; Cheng et al., 1996; Cui et al., 2002; Erill et al., 2003; Krishnan et al., 2004a,b; Lee et al., 2003a,b,c, 2000; Lin et al., 2000a,b,c, 2002; Pal and Venkataraman, 2002; Rodriguez et al.,…”