CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design

By T. Rose, Jorja G. Henikoff, Steven Henikoff. Nucleic Acids Res., 2003

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Sensitive, Seminested PCR Amplification of VP1 Sequences for Direct Identification of All Enterovirus Serotypes from Original Clinical Specimens (2006, methodology): “Since its initial description (30), the CODEHOP approach has been used to amplify previously unknown gene homologs in a wide variety of plants, animals, and bacteria, as well as herpesviruses and retroviruses (29).”
A distinct lineage of influenza A virus from bats (2012, positive): “Consensus-degenerate RT-PCR/PCR primers designed to identify the most conserved genomic sequence regions of virus families, subfamilies, or genera is a powerful technology for the detection of novel pathogens (10) and has been used to identify a number of new viruses (11–13).”