Evaluating Seed Viability by an Unimbibed Seed Crush Test in Comparison with the Tetrazolium Test1

By Jeremiah T. Sawma, C. L. Mohler. Weed technology, 2002

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Seed mortality in the soil is related to seed coat thickness (2010, background): “Finally, any remaining ungerminated seeds were dissected under a microscope, and firm white embryos were considered as viable (Sawma and Mohler, 2002).”
The role of seedlings and seed bank viability in the recovery of Chesapeake Bay, USA, Zostera marina populations following a large-scale decline (2010, methodology): “The sediment was stored at 4 C until analysis which was usually within 48 h. Seeds were sieved from the sediment (0.5 mm mesh) and then tested for viability using the tetrazolium staining method (Lakon, 1949; AOSA, 1981; Sawma & Mohler, 2002).”
Comparing Estimates of Seed Viability in Three Foxtail (Setaria) Species Using the Imbibed Seed Crush Test with and Without Additional Tetrazolium Testing (2007, methodology): “However, there is some debate as to the reliability of the tetrazolium test (Mohamed et al. 1998; Sawma and Mohler 2002).”