Physical characterization and diel dynamics of different fractions of extracellular polysaccharides in an axenic culture of a benthic diatom

By J. de Brouwer, K. Wolfstein, L. Stal. 2002

99 citations recorded by the source corpus.

Subfields: Biomaterials, Environmental Chemistry, Pollution

View Paper: https://www.semanticscholar.org/paper/0dcd69ec46c1a95ceca5222c4ffd52605f5b7d45

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Citations in the corpus

Optimising extraction of extracellular polymeric substances (EPS) from benthic diatoms: comparison of the efficiency of six EPS extraction methods (2009, methodology): “…1 hour (Chiovitti et al. 2003; 2004), whilst a strain of Cylindrotheca closterum showed little evidence of cell lysis with this extraction method (de Brouwer et al. 2002). de Brouwer and Stal (2004) argued that even if there is evidence of cell lysis and release from intracellular glucans with the…”
ENVIRONMENTAL EFFECTS ON EXOPOLYMER PRODUCTION BY MARINE BENTHIC DIATOMS: DYNAMICS, CHANGES IN COMPOSITION, AND PATHWAYS OF PRODUCTION 1 (2004, background): “Higher cell-normalized rates of production of a cell-associated EPS during nutrient-replete compared with nutrient-limited conditions have also been reported for C. closterium (de Brouwer et al. 2002).”
The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides (2004, methodology): “The discrepancy in the results of the DiBAC4(3) labelling between the present study and the experiments on C. closterium reported by de Brouwer et al. (2002) may partly be due to differences in the staining intensity regarded by the respective workers as positive or negative.”
EXTRACELLULAR MATRIX ASSEMBLY IN DIATOMS (BACILLARIOPHYCEAE). V. ENVIRONMENTAL EFFECTS ON POLYSACCHARIDE SYNTHESIS IN THE MODEL DIATOM, PHAEODACTYLUM TRICORNUTUM 1 (2006, result): “This is in agreement with de Brouwer et al. (2002) and Underwood et al. (2004) who reported enhanced EPS production cell 1 in Cylindrotheca closterium during logarithmic growth (compared with stationary growth).”
Influence of temperature on the relationship between oxygen- and fluorescence-based estimates of photosynthetic parameters in a marine benthic diatom (Cylindrotheca closterium) (2003, methodology): “…was grown in continuous culture [turbidostat mode] in nutrient replete modified F/2 medium (Guillard & Ryther 1962, modified according to De Brouwer et al. 2002) at an average incident irradiance of 300 µmol m P -2 P sP -1 Pin the ‘high’ growth rate culture and 200 µmol mP -2 P s P -1 P in…”
Effect of UV stress on the fatty acid and lipid class composition in two marine microalgae Pavlova lutheri (Pavlovophyceae) and Odontella aurita (Bacillariophyceae) (2010, methodology): “et al. 2007) according to Cullen et al. (1992) ).”
Light response curve methodology and possible implications in the application of chlorophyll fluorescence to benthic diatoms (2006, background): “The original medium was 118 complemented following De Brouwer et al. (2002), with the addition of Fe-NH4-119 citrate (1.37 µM final concentration), CuSO4 5H2O (0.04 µM f.c.), folic acid (0.18 nM 120 f.c.), nicotinic acid (0.0325 µM f.c.), thymine (0.95 µM f.c.), Ca-d-pantothenate (8.39 121 nM f.c.)…”
Microphytobenthos, their Extracellular Polymeric Substances, and the Morphogenesis of Intertidal Sediments (2003, methodology): “In the presence of high amounts of divalent cations (Ca2+, Mg2+) and charged silt and clay particles, this EPS becomes tightly bound to the sediment and can only be dissolved after chelating the cations using high concentrations of EDTA (de Brouwer et al. 2002a).”
The carbohydrates of Phaeocystis and their degradation in the microbial food web (2007, background): “b-1,3-glucans have an essentially linear b-D-1,3-glucan backbone with some branching at position 6 or 2 (Percival 1970; Craigie 1974; Myklestad 1978; Paulsen and Myklestad 1978).”