Seasonal dynamics and phylogenetic diversity of free-living and particle-associated bacterial communities in four lakes in northeastern Germany

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Spatiotemporal dynamics of bacterial community composition in large shallow eutrophic Lake Taihu: High overlap between free‐living and particle‐attached assemblages (2017, methodology): “…sequentially filtered through 5.0-lm, 2-lm, and 0.2-lm polycarbonate membranes (47- mm diameter, Millipore), respectively, under the vacuum pressure of <20 mm Hg (Allgaier and Grossart 2006; Parveen et al. 2011) to separate particle-attached (PA, 5.0 lm) and free-living bacteria (FL, 0.2–2 lm).”
Coupling of heterotrophic bacteria to phytoplankton bloom development at different pCO 2 levels: a mesocosm study (2008, methodology): “DNA fragments were sequenced as described previously (Allgaier and Grossart, 2006a) using primers 341f and 907r. GelCompar II first a band based binary presence/absence table was calculated applying Dice similarity coefficient.”