Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca

By K. Kao. Molecular and General Genetics MGG, 2004

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In vitro fertilization of single, isolated gametes of maize mediated by electrofusion (2004, methodology): “Embryos with developing callus were individually cultivated in macroplates for up to 7 months in 2 ml liquid Kao medium (Kao 1977) following the modifications of Nakamura and Keller (1982) and using the micronutrient composition of Schenk and Hildebrandt (1972) supplemented with 8 mg/1 2,4-D, pH 5.”
Intergeneric somatic hybridization and its application to crop genetic improvement (2005, background): “Verification of hybrids via chromosome morphology difference has been used in characterizing a number of somatic hybrids (Kao, 1977; Binding and Nehls, 1978; Chien et al., 1982; Gleba et al., 1982, 1988; Gupta et al., 1984; Kushnir et al., 1987; Endo et al., 1988; Hinnisdaels et al., 1991; Kostenyuk”
Morphological characteristics and chloroplast DNA distribution in different cytoplasmic parasexual hybrids of Nicotiana tabacum (2004, background): “Most of the previous reports deal with the fate of the parental nuclei and chromosomes both during culture of hybrid cell lines and plant regeneration (Constabel etal. , 1976; Dudits etal. , 1976; Kao, 1977; Melchers and Sacrist~m, 1977).”