A promoter-level mammalian expression atlas

By The Fantom Consortium, Riken Pmii, Riken Clst, A. Forrest, H. Kawaji, M. Rehli, J. Baillie, M. D. de Hoon, Vanja Haberle, Timo Lassmann and 254 more. Nature, 2014

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Regulated transcription controls the diversity, developmental pathways and spatial organization of the hundreds of cell types that make up a mammal. Using single-molecule cDNA sequencing, we mapped transcription start sites (TSSs) and their usage in human and mouse primary cells, cell lines and tissues to produce a comprehensive overview of mammalian gene expression across the human body. We find that few genes are truly ‘housekeeping’, whereas many mammalian promoters are composite entities composed of several closely separated TSSs, with independent cell-type-specific expression profiles. TSSs specific to different cell types evolve at different rates, whereas promoters of broadly expressed genes are the most conserved. Promoter-based expression analysis reveals key transcription factors defining cell states and links them to binding-site motifs. The functions of identified novel transcripts can be predicted by coexpression and sample ontology enrichment analyses. The functional annotation of the mammalian genome 5 (FANTOM5) project provides comprehensive expression profiles and functional annotation of mammalian cell-type-specific transcriptomes with wide applications in biomedical research.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/0b83e0898175af57a67aa23427b520ea984d51bc

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In Molecular Biology

Citations in the corpus

The Ensembl Regulatory Build (2015, background): “Of the 217,516 strict TSS calls found with CAGE tags by the FANTOM 5 consortium [31], 88.9% were recovered.”