Development of Dominant Rice Blast Pi‐ta Resistance Gene Markers
By Yulin Jia, Zhonghua Wang, Pratibha Singh. Crop science, 2002
146 citations recorded by the source corpus.
Incorporation of resistance genes into existing rice (Oryza sativa L.) cultivars is a powerful strategy and is commonly applied in breeding rice resistance to blast disease [caused by Pyricularia grisea Sacc. = P. oryzae Cavara (teleomorph: Magnaporthe grisea (Hebert) Barr)]. The rice blast resistance gene, Pi-ta, originally introgressed into japonica from indica rice is important in breeding for rice blast resistance worldwide. In the southern USA, the rice cultivar Katy contains Pi-ta and is resistant to the predominant blast M. grisea races IB-49 and IC-17 and has been used as the blast resistant breeding parent. Three pairs of DNA primers specific to the dominant indica Pi-ta gene were designed to amplify the Pi-ta DNA fragments by polymerase chain reaction (PCR). PCR products amplified by these Pi-ta specific primers were cloned and sequenced. Sequence analysis confirmed the presence of the dominant indica Pi-ta allele. These Pi-ta primers were used to examine the presence of Pi-ta alleles in advanced Arkansas rice breeding lines. The Pi-ta containing rice lines, as determined by PCR analysis, were resistant to both IB-49 and IC-17 in standard pathogenicity assays. In contrast, lines lacking the Pi-ta genes failed to protect rice plants against both races IB-49 and IC-17. The presence of Pi-ta markers correlated with the Pi-ta resistance spectrum. Thus, the Pi-ta gene markers provide a basis for stacking other blast resistance genes into high yielding and good quality advanced breeding rice lines.
Subfields: Cell Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/0ab667410a12a641ad29589861529d0f7a67ae75
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In Plant Science
Citations in the corpus
- Development of DNA Markers Suitable for Marker Assisted Selection of Three Pi Genes Conferring Resistance to Multiple Pyricularia grisea Pathotypes (2004, background): “*Corresponding author (r-fjellstrom@tamu.edu). et al., 2000) and Pi-ta (Jia et al., 2002).”
- Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54 (Pikh) (2010, background): “This can be explained by the fact that the two IR cultivars are known to possess Pita and Pib genes (Ebron et al. 2004) and Palamdhan 957 possesses the Pi2 gene (based on marker analysis carried out in our laboratory).”