Construction of a genetic toggle switch in Escherichia coli

By T. Gardner, C. Cantor, James J. Collins. Nature, 2000

4,394 citations recorded by the source corpus.

Subfields: Astronomy and Astrophysics, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/0a897f0e7adc1dfd39ff5fdb2cab46ecf08e5015

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Citations in the corpus

Fast Numerical Methods for Stochastic Computations: A Review (2009, result): “The re-production of the experimental results from [22] is courtesy of Dr.”
Gene regulation: Towards a circuit engineering discipline (2000, background): “[3] demonstrate how this inherently bistable configuration can be engineered so that external signals can flip the system from one state to the other.”
Fokker–Planck approximation of the master equation in molecular biology (2009, background): “This motif has been implemented in vivo in a cell [14].”
Synthetic biology - putting engineering into biology (2006, methodology): “Ideal, however, would be models that allow deriving in silico suggestions for optimal design strategies or debugging, prior to implementation of the circuit in vivo ( Gardner, et al., 2000; Sprinzak and Elowitz, 2005).”
Stochasticity in transcriptional regulation: origins, consequences, and mathematical representations. (2001, background): “In fact, however, consideration of the detailed mechanism of transcriptional regulation and of observations of cellular phenomena governed by transcriptional regulation indicate the presence of significant stochasticity (Ahmad and Henikoff, 2001; Gardner et al. 2000; Grossman, 1995; Thattai, 2001).”
Stochastic gene expression and its consequences (2008, background): “This bistable expression pattern has been observed in synthetic systems with positive feedback switches (Becskei et al., 2001; Gardner et al., 2000; Isaacs et al., 2003; Kramer and Fussenegger, 2005) and also in several”
A Synthetic Genetic Edge Detection Program (2009, background): “Individual genetic circuits that mimic basic electronic functions have previously been constructed (Anderson et al., 2007; Atkin- son et al., 2003; Basu et al., 2004; Elowitz and Leibler, 2000; Gardner et al., 2000; Guet et al., 2002; Ham et al., 2006; Yoko- bayashi et al., 2002).”
Negative autoregulation speeds the response times of transcription networks. (2002, background): “The delay leads to a decrease in the rise time in comparison to the model with no delay (equations (9) and ( 10 ) in Materials and Methods), marked by T ¼ 0i nFigure 2. The bold line shows cases where an overshoot is obtained.”
Nonequilibrium physics in biology (2019, background): “The toggle switch constructed by Collins and colleagues (Gardner et al., 2000) is a simplified version of the lambda phage switch previously discussed.”