Phyllotreta striolata (Coleoptera: Chrysomelidae): Arginine kinase cloning and RNAi-based pest control
By Yiying Zhao, Guang Yang, G. Wang-Pruski, Minsheng You. 2008
90 citations recorded by the source corpus.
Insect pests cause billions of dollars in crop losses and there is the ever-present threat of insecticide resistance, pesticide pollution of food and environmental damage. New ways of controlling insect pests are urgently needed. Arginine kinase (AK) is a phosphotransferase, which plays a critical role in cellular energy metabolism in invertebrates. It only presents in invertebrates and may be a suitable chemotherapeutic target in the control of pests. In this study, we cloned and characterized the full-length AK gene from Phyllotreta striolata, one of the most destructive beetle pests worldwide. Furthermore, we constructed a dsRNA targeting AK and used RNAi to control the beetle. The feeding bioassays indicated that minute quantities of dsRNA greatly impaired the beetle's development. Ingestion of dsRNA not only significantly retarded the development and increased the mortality of adults, it also greatly reduced fecundity and fertility, suggesting that RNAi targeting AK is a potential and attractive tool for controlling insect pests.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/09f2e08400f4da105eee5e4faecd38e8839e93ed
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In Molecular Biology
Citations in the corpus
- Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review. (2010, background): “fecundity and fertility Zhao et al. (2008)”
- Chapter Five – Progress Towards RNAi-Mediated Insect Pest Management (2014, background): “This sensitivity to environmental RNAi extends to both the larval and adult stages (Rangasamy and Siegfried, 2012; Zhao et al., 2008).”