Adaptive laboratory evolution – principles and applications for biotechnology

By Martin Dragosits, Diethard Mattanovich. Microbial Cell Factories, 2013

721 citations recorded by the source corpus.

Adaptive laboratory evolution is a frequent method in biological studies to gain insights into the basic mechanisms of molecular evolution and adaptive changes that accumulate in microbial populations during long term selection under specified growth conditions. Although regularly performed for more than 25 years, the advent of transcript and cheap next-generation sequencing technologies has resulted in many recent studies, which successfully applied this technique in order to engineer microbial cells for biotechnological applications. Adaptive laboratory evolution has some major benefits as compared with classical genetic engineering but also some inherent limitations. However, recent studies show how some of the limitations may be overcome in order to successfully incorporate adaptive laboratory evolution in microbial cell factory design. Over the last two decades important insights into nutrient and stress metabolism of relevant model species were acquired, whereas some other aspects such as niche-specific differences of non-conventional cell factories are not completely understood. Altogether the current status and its future perspectives highlight the importance and potential of adaptive laboratory evolution as approach in biotechnological engineering.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/09e8fffffe29d6b53bee072e4f632e32239a43f8

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Citations in the corpus

The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology (2019, background): “…al., 2018; Williams 29 et al., 2016) and tolerance (Peabody et al., 2014), as well as other industrially relevant 30 strain properties (Bachmann et al., 2015; Dragosits and Mattanovich, 2013; Portnoy et 31 5 al., 2011/8; Stella et al., 2019; Winkler and Kao, 2014; Fernández-Cabezón et al., 1 2019).”
Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose (2024, neutral): “Microbiota species/ strain-speci ﬁ c induction or suppression of MHC class II expression on intestinal epithelial cells modulates cognate antigen availability to T cells, affecting aGVHD initiation and severity 11 – 13 .”
Engineering Cellular Metabolism (2008, positive): “, 2005), as well as for improving the tolerance to harsh conditions or to the product of interest, as reviewed recently (Dragosits and Mattanovich, 2013).”