Baculovirus as versatile vectors for protein expression in insect and mammalian cells
By T. Kost, J. Condreay, D. Jarvis. Nature Biotechnology, 2005
1,010 citations recorded by the source corpus.
Today, many thousands of recombinant proteins, ranging from cytosolic enzymes to membrane-bound proteins, have been successfully produced in baculovirus-infected insect cells. Yet, in addition to its value in producing recombinant proteins in insect cells and larvae, this viral vector system continues to evolve in new and unexpected ways. This is exemplified by the development of engineered insect cell lines to mimic mammalian cell glycosylation of expressed proteins, baculovirus display strategies and the application of the virus as a mammalian-cell gene delivery vector. Novel vector design and cell engineering approaches will serve to further enhance the value of baculovirus technology.
Subfields: Biotechnology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/096a093c75831eb707101003e715240745e6a334
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Citations in the corpus
- Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors (2011, neutral): “However, insect cell line-based platforms can be cumbersome to implement and do not correctly recapitulate complex mammalian N-glycans containing galactose or sialic acid residues (5,6).”
- Baculovirus Pesticides: Present State and Future Perspectives (2011, background): “Though baculoviruses can enter mammalian cells, productive viral infection does not occur even at very high multiplicity of infection (Kost et al. 2005).”