Visualization of cortical, subcortical, and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses
By Shanna Resendez, Joshua H. Jennings, Randall L. Ung, V. Namboodiri, Z. Zhou, James M. Otis, H. Nomura, J. McHenry, O. Kosyk, Garret D. Stuber. Nature Protocols, 2016
295 citations recorded by the source corpus.
This protocol describes how to use miniature, integrated microscopes in conjunction with an implantable microendoscopic lens to guide light into and out of the brain, thereby enabling optical access to the brain. Genetically encoded calcium indicators for visualizing dynamic cellular activity have greatly expanded our understanding of the brain. However, owing to the light-scattering properties of the brain, as well as the size and rigidity of traditional imaging technology, in vivo calcium imaging has been limited to superficial brain structures during head-fixed behavioral tasks. These limitations can now be circumvented by using miniature, integrated microscopes in conjunction with an implantable microendoscopic lens to guide light into and out of the brain, thus permitting optical access to deep brain (or superficial) neural ensembles during naturalistic behaviors. Here we describe steps to conduct such imaging studies using mice. However, we anticipate that the protocol can be easily adapted for use in other small vertebrates. Successful completion of this protocol will permit cellular imaging of neuronal activity and the generation of data sets with sufficient statistical power to correlate neural activity with stimulus presentation, physiological state and other aspects of complex behavioral tasks. This protocol takes 6–11 weeks to complete.
Subfields: Biophysics, Cellular and Molecular Neuroscience, Cognitive Neuroscience
View Paper: https://www.semanticscholar.org/paper/08a945d4dfb3392ea06bd2562542a0c5167fc4d0
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Citations in the corpus
- Tracking the Same Neurons across Multiple Days in Ca2+ Imaging Data (2017, background): “Thus, the set of detected cells can differ between sessions (Ziv et al., 2013; Resendez et al., 2016; Grewe et al., 2017; Xia et al., 2017), and a one-toone mapping of neural identity across sessions is typically not attainable.”
- Efficient and accurate extraction of in vivo calcium signals from microendoscopic video data (2018, unknown): “Unfortunately, both approaches have well-known flaws (Resendez et al., 2016).”