Probing Gene Expression in Live Cells, One Protein Molecule at a Time

By Ji Yu, Jie Xiao, X. Ren, K. Lao, Xiaohui S. Xie. Science, 2006

948 citations recorded by the source corpus.

Subfields: Biophysics, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/078c58a4dcb283b5086863871e06d6ce2d32dca8

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Citations in the corpus

A Whole-Cell Computational Model Predicts Phenotype from Genotype (2012, neutral): “We found that the model calculations were consistent with the observed doubling time (Figures 2A and 2B), cellular chemical composition (Figure 2C), replication of major cell mass fractions (Figure 2D), and gene expression ( R 2 = 0.68; Figure S1A).”
Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “For example, fast-maturing yellow FPs allowed the direct observation of the production of a single protein molecule in real time (503).”
Analytical distributions for stochastic gene expression (2008, methodology): “Such bursts of gene expression have been measured in bacteria and eukaryotes [12, 13, 14, 28, 29].”
Single-RNA counting reveals alternative modes of gene expression in yeast (2008, background): “Notably, bursting as well as constitutive RNA expression have been described in bacteri”
Mass production and dynamic imaging of fluorescent nanodiamonds. (2008, background): “Compared with organic fluorophores whose time-lapse imaging is limited by photobleachin”
SINGLE-PARTICLE TRACKING (2009, background): “[28] observed directly the production of a single protein in an individual cell in real time.”
Multicolor Conjugated Polymer Dots for Biological Fluorescence Imaging (2008, background): “Green fluorescent proteins (GFP) and some derivatives can in favorable cases be detected at the single molecule level.(7) However, approximately 30 copies of GFP are required for long-term single molecule tracking inside cells.”
Stochastic gene expression and its consequences (2008, methodology): “(2006) used two methods: one involving microfluidics, in which they quantified the number of beta-galactosidase enzymes in a cell by monitoring its enzymatic activity, and one involving direct visualization of single YFP molecules tethered to the cellular membrane (Yu et al., 2006).”
Stochastic switching in biology: from genotype to phenotype (2017, methodology): “For example Yu et al [256] used fluorescent imaging to observe the expression of individual protein molecules in single Escerichia cells under the control of a repressed lac J.”