Adaptive optical microscopy: the ongoing quest for a perfect image

By Martin Booth. Light: Science & Applications, 2014

580 citations recorded by the source corpus.

Adaptive optics is becoming a valuable tool for high resolution microscopy, providing correction for aberrations introduced by the refractive index structure of specimens. This is proving particularly promising for applications that require images from deep within biological tissue specimens. We review recent developments in adaptive microscopy, including methods and applications. A range of advances in different microscope modalities is covered and prospects for the future are discussed. Adaptive optics is used to improve image quality across a wide range of microscopy techniques. Martin Booth from the University of Oxford in the UK reviews how technologies such as deformable mirrors and spatial light modulators, which compensate for aberrations by locally controlling the wavefront of a light wave, are now improving the performance of multiphoton, confocal, widefield and super-resolution microscopes. The benefits of such improvements are especially appealing for images captured from within biological tissue (focal distances of tens to hundreds of micrometres), where low-order aberrations associated with smooth phase variations occur. One future challenge is the development of efficient measurement and correction schemes for higher-order phase variations.

Subfields: Atomic and Molecular Physics, and Optics, Biomedical Engineering, Biophysics

View Paper: https://www.semanticscholar.org/paper/06cfdf1b6b85bbae4930c92b404d661382055d05

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In Atomic and Molecular Physics, and Optics

In Biomedical Engineering

In Biophysics

Citations in the corpus

Roadmap on wavefront shaping and deep imaging in complex media (2021, background): “Additionally, adaptive optics (AO), which is a well-established technique for improving the spatial resolution and increasing the signal generation for in vivo brain imaging [25, 26], may be considered.”