Over-accumulation of astaxanthin in Haematococcus pluvialis through chloroplast genetic engineering
By Janeth I. Galarza, J. Gimpel, Verónica Rojas, B. Arredondo-Vega, V. Henríquez. 2018
111 citations recorded by the source corpus.
Abstract The carotenoid biosynthesis pathway of Haematococcus pluvialis has been genetically modified to overproduce astaxanthin, a red pigment of high commercial value. The endogenous phytoene desaturase coding sequence (pds) has been codon optimized and overexpressed in the chloroplast of H. pluvialis under the control of the psbA promoter/UTR. After biolistic transformation and selection, PDS mRNA and protein were detected in transformants. Astaxanthin accumulated up to 67% higher in transformed strains than in wild-type upon induction with high light intensity and nitrogen depletion. This is the first report of plastid transformation of a microalgae with its endogenous pds nuclear gene.
Subfields: Biochemistry, Molecular Biology, Renewable Energy, Sustainability and the Environment
View Paper: https://www.semanticscholar.org/paper/06c7fd1766f037b4bd1ecf7875870d873f539d3a
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In Biochemistry
In Molecular Biology
In Renewable Energy, Sustainability and the Environment
Citations in the corpus
- Variables Governing Photosynthesis and Growth in Microalgae Mass Cultures (2021, methodology): “Significant enhancements in carotenoid productivity were obtained by modification of the astaxanthin synthesis pathway—either over-expressing the β-carotene ketolase, or by expressing the endogenous phytoene desaturase in the chloroplast [161,162].”