An in vivo model of functional and vascularized human brain organoids
By A. Mansour, J. Gonçalves, Cooper W Bloyd, Hao Li, Sarah Fernandes, Daphne Quang, Stephen T. Johnston, Sarah L. Parylak, Xin Jin, Fred H. Gage. Nature Biotechnology, 2018
1,043 citations recorded by the source corpus.
Differentiation of human pluripotent stem cells to small brain-like structures known as brain organoids offers an unprecedented opportunity to model human brain development and disease. To provide a vascularized and functional in vivo model of brain organoids, we established a method for transplanting human brain organoids into the adult mouse brain. Organoid grafts showed progressive neuronal differentiation and maturation, gliogenesis, integration of microglia, and growth of axons to multiple regions of the host brain. In vivo two-photon imaging demonstrated functional neuronal networks and blood vessels in the grafts. Finally, in vivo extracellular recording combined with optogenetics revealed intragraft neuronal activity and suggested graft-to-host functional synaptic connectivity. This combination of human neural organoids and an in vivo physiological environment in the animal brain may facilitate disease modeling under physiological conditions.
Subfields: Biomedical Engineering, Developmental Neuroscience, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/06c3bbb1eb9c1edea741856e8da328f54de2c9b3
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In Developmental Neuroscience
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Citations in the corpus
- Brain organoids: advances, applications and challenges (2019, background): “The transplantation of organoids into animals, allowing the host vasculature to grow into the organoid graft, has also proven to be a promising approach to promote organoid viability and maturation (Mansour et al., 2018).”