Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients
By Y. Xiong, Yuan Liu, Liu Cao, Dehe Wang, M. Guo, Ao Jiang, Dong Guo, Wenjia Hu, Jiayi Yang, Zhidong Tang and 9 more. Emerging Microbes and Infections, 2020
1,034 citations recorded by the source corpus.
ABSTRACT Circulating in China and 158 other countries and areas, the ongoing COVID-19 outbreak has caused devastating mortality and posed a great threat to public health. However, efforts to identify effectively supportive therapeutic drugs and treatments has been hampered by our limited understanding of host immune response for this fatal disease. To characterize the transcriptional signatures of host inflammatory response to SARS-CoV-2 (HCoV-19) infection, we carried out transcriptome sequencing of the RNAs isolated from the bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) specimens of COVID-19 patients. Our results reveal distinct host inflammatory cytokine profiles to SARS-CoV-2 infection in patients, and highlight the association between COVID-19 pathogenesis and excessive cytokine release such as CCL2/MCP-1, CXCL10/IP-10, CCL3/MIP-1A, and CCL4/MIP1B. Furthermore, SARS-CoV-2 induced activation of apoptosis and P53 signalling pathway in lymphocytes may be the cause of patients’ lymphopenia. The transcriptome dataset of COVID-19 patients would be a valuable resource for clinical guidance on anti-inflammatory medication and understanding the molecular mechansims of host response.
Subfields: Infectious Diseases, Neurology
View Paper: https://www.semanticscholar.org/paper/06aeca807f0ef7c563924b5b579beaf526a2a789
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Citations in the corpus
- Cytokine Storm in COVID-19: The Current Evidence and Treatment Strategies (2020, background): “Artesunate can lower levels of IL-1β, IL-2, IL-6, IFN-γ, CCL2, CCL3, and CCL4 in these mice.”
- Should we stimulate or suppress immune responses in COVID-19? Cytokine and anti-cytokine interventions (2020, positive): “Intriguingly, while being central to cytokine storm occurrence in SARS-CoV infection [148], IFN-γ was not found to be elevated in the sera or BALFs of patients with severe forms of COVID-19 [22,23,26,149].”
- Immune Response, Inflammation, and the Clinical Spectrum of COVID-19 (2020, methodology): “(127) used RNA-seq to study BALFs and peripheral blood mononuclear cells (PBMC) transcriptome from three COVID-19 patients (unfortunately their clinical conditions were not reported) and from three healthy subjects.”
- SARS-CoV-2 infection: The role of cytokines in COVID-19 disease (2020, neutral): “detected elevated levels of MCP-1 and other cytokines in the bronchoalveolar lavage fluid of patients with COVID19 and associated the pathogeny of the virus with these cytokines [186].”