Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram‐positive bacteria

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Generating functional protein variants with variational autoencoders (2020, methodology): “The variants were amplified from synthesized DNA fragments using primers F342/F343 493 and cloned under the control of the T7 promoter and upstream of C-terminal His- Tag sequence 494 in plasmid pET28/16 [53] amplified with primers F340/F341 through Gibson assembly [54].”
New Vector for Efficient Allelic Replacement in Naturally Nontransformable, Low-GC-Content, Gram-Positive Bacteria (2004, methodology): “1A): (i) a NaeI- BglII DNA fragment containing the extended multiple cloning site from the pMTL22 plasmid (3); (ii) a chromosomal BglIIBamHI DNA fragment (128 bp) containing the constitutively expressed promoter region of the S. aureus clpB gene encoding a Clp-Hsp100 ATPase (5, 6) and amplified by P”