A Heat-Inducible Transcription Factor, HsfA2, Is Required for Extension of Acquired Thermotolerance in Arabidopsis1[W][OA]
By Y. Charng, Hsiang-Chin Liu, Nai-yu Liu, Wen-tzu Chi, Chun-Neng Wang, Shih-hsun Chang, Tsu-Tsuen Wang. Plant Physiology, 2006
610 citations recorded by the source corpus.
The expression of heat shock proteins (Hsps) induced by nonlethal heat treatment confers acquired thermotolerance (AT) to organisms against subsequent challenges of otherwise lethal temperature. After the stress signal is removed, AT gradually decays, with decreased Hsps during recovery. AT of sufficient duration is critical for sessile organisms such as plants to survive repeated heat stress in their environment, but little is known regarding its regulation. To identify potential regulatory components, we took a reverse genetics approach by screening for Arabidopsis (Arabidopsis thaliana) T-DNA insertion mutants that show decreased thermotolerance after a long recovery (2 d) under nonstress conditions following an acclimation heat treatment. Among the tested mutants corresponding to 48 heat-induced genes, only the heat shock transcription factor HsfA2 knockout mutant showed an obvious phenotype. Following pretreatment at 37°C, the mutant line was more sensitive to severe heat stress than the wild type after long but not short recovery periods, and this could be complemented by the introduction of a wild-type copy of the HsfA2 gene. Quantitative hypocotyl elongation assay also revealed that AT decayed faster in the absence of HsfA2. Significant reduction in the transcript levels of several highly heat-inducible genes was observed in HsfA2 knockout plants after 4 h recovery or 2 h prolonged heat stress. Immunoblot analysis showed that Hsa32 and class I small Hsp were less abundant in the mutant than in the wild type after long recovery. Our results suggest that HsfA2 as a heat-inducible transactivator sustains the expression of Hsp genes and extends the duration of AT in Arabidopsis.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/0631ec8db5a9fd9fcfc84c906f0443ebfe4c8781
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Citations in the corpus
- The heat‐shock protein/chaperone network and multiple stress resistance (2017, background): “They are responsible for the heat-acclimation phenotype (Charng et al., 2006; Nishizawa et al., 2006; Schramm et al., 2008).”
- Plant heat-shock proteins: A mini review (2011, neutral): “These factors have been classified (Tripp et al., 2009) into three classes according to the structural differences in their aggregation in triples, i.e. oligomerization domains as follows: Plant HsfA such as HsfA1 and HsfA2 in L. esculentum Plant HsfB such as HsfB1 in L. esculentum Plant HsfC Each fa”