DNA shuffling of a family of genes from diverse species accelerates directed evolution

By A. Crameri, S. Raillard, Ericka Bermudez, W. Stemmer. 1998

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Citations in the corpus

Exploring protein fitness landscapes by directed evolution (2009, positive): “Naturally-occurring homologous proteins can be recombined to create genetic diversity within protein sequence librarie”
Directed Polymerase Evolution (2013, methodology): “Family shuffling has proven to be an effective way to construct high-quality libraries; however, reassembly is relatively inefficient when the method is applied to regions with low sequence homology [45].”
Programming cells by multiplex genome engineering and accelerated evolution (2009, background): “7); (2) induce cells for allelic replacement via 42 uC heat shock for 15 min; (3) chill cells at 4 uC to halt cellular metabolism; (4) wash cells through 15–20 iterations of filtration and resuspension with dH2O; (5) mix cell suspension and synthetic DNA; (6) deliver DNA into cells by electroporatio”
Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes (2009, background): “For example, libraries can be constructed from pools of DNaseI digested fragments prepared from parental templates [5,6,7,8], from degenerate oligonucleotides [9,10] or from mixtures of both, or even from undigested parental templates [11,12,13], and are usually assembled through PCR.”
Directed evolution of new catalytic activity using the α/β-barrel scaffold (2000, background): “, the generation of mutants must be coupled to a suitable selection procedure to allow in vitro evolutio”
PERSPECTIVE: TRANSPOSABLE ELEMENTS, PARASITIC DNA, AND GENOME EVOLUTION (2001, background): “Experimental evolution (Moore et al. 1997; Zhang et al. 1997; Crameri et al. 1998) and computer simulation experiments (Bogarad and Deem 1999) showed that sequence shuffling has the potential to improve protein function significantly better than does point mutation alone.”
Improving catalytic function by ProSAR-driven enzyme evolution (2007, methodology): “The iterative nature of this approach results in stepwise improvements in overall function, yielding substantial improvements in desired enzyme propertie”
In silico protein design by combinatorial assembly of protein building blocks (2004, methodology): “Recombination is a powerful tool for the engineering and optimization of proteins in vitro (Crameri et al. 1998; Riechmann and Winter 2000).”
Protein building blocks preserved by recombination (2002, background): “It promotes the combination of traits from multiple parents onto a single offspring, thus exploiting information obtained in previous rounds of selectio”
Adeno-associated virus serotypes: vector toolkit for human gene therapy. (2006, background): “Template switching causes crossovers in areas of sequence homology [117].”