Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid

By L. Huan, T. Takamura, Michio Tanaka. 2004

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The Chemical Composition and Biological Properties of Coconut (Cocos nucifera L.) Water (2009, background): “They are mostly used to propagate protocorm-like bodies of orchids in plant industries [49].”
Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl. (2008, background): “steps of PLB regeneration in orchids (Steward and Mapes 1971; Kerbauy 1984; Begum et al. 1994; Chen and Chang 2000; Huan et al. 2004).”
Priming biotic factors for optimal protocorm-like body and callus induction in hybrid Cymbidium (Orchidaceae), and assessment of cytogenetic stability in regenerated plantlets (2006, background): “, 1966; Ueda and Torikata, 1968; Kim and Kako, 1984) and PLBs (Begum et al., 1994b; Huan and Tanaka, 2004a, 2004b; Huan et al., 2004) have been described.”
Direct and callus-mediated protocorm-like body induction from shoot-tips of Dendrobium chrysotoxum Lindl. (Orchidaceae) (2007, background): “Conversely, the callus cultures of most orchids exhibit an absolute requirement of both auxin and cytokinin for their maintenance (Chen and Chang 2000; Lin et al. 2000; Lee and Lee 2003; Huan and Tanaka 2004; Huan et al. 2004).”
The Application of Biotechnology to Orchids (2013, background): “However, the combinations, concentrations, and the ratio of plant growth regulators (PGRs) are critically important, Figure 2 (Teng et al., 1997; Huan et al., 2004).”