Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis.

By R. Strasser, M. Tsimilli-Michael, S. Qiang, Vasilij Goltsev. Biochimica et Biophysica Acta, 2010

679 citations recorded by the source corpus.

A new instrument (M-PEA), which measures simultaneously kinetics of prompt fluorescence (PF), delayed fluorescence (DF) and modulated light reflection at 820nm (MR), was used to screen dark-adapted leaves of the resurrection plant Haberlea rhodopensis during their progressive drying, down to 1% relative water content (RWC), and after their re-watering. This is the first investigation using M-PEA, which employs alternations of actinic light (627-nm peak, 5000 micromol photons m(-2) s(-1)) and dark intervals, where PF-MR and DF kinetics are respectively recorded, with the added advantages: (a) all kinetics are recorded with high time resolution (starting from 0.01 ms), (b) the dark intervals' duration can be as short as 0.1 ms, (c) actinic illumination can be interrupted at different times during the PF transient (recorded up to 300 s), with the earliest interruption at 0.3 ms. Analysis of the simultaneous measurements at different water-content-states of H. rhodopensis leaves allowed the comparison and correlation of complementary information on the structure/function of the photosynthetic machinery, which is not destroyed but only inactivated (reversibly) at different degrees; the comparison and correlation helped also to test current interpretations of each signal and advance their understanding. Our results suggest that the desiccation tolerance of the photosynthetic machinery in H. rhodopensis is mainly based on mechanism(s) that lead to inactivation of photosystem II reaction centres (transformation to heat sinks), triggered already by a small RWC decrease.

Subfields: Global and Planetary Change, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/0319a1ac87ceca19020d7fbb7bcee914221ccf27

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Citations in the corpus

PSII Fluorescence Techniques for Measurement of Drought and High Temperature Stress Signal in Crop Plants: Protocols and Applications (2013, background): “The chlorophyll fluorescence emitted by higher plants upon illumination carries a lot of information about the structure and function of the photosynthetic apparatus (Strasser et al. 2010).”
Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions (2016, background): “ChlF is emitted following a dark-to-light transition of a photosynthetic sample, while delayed fluorescence emission (DF) occurs during light-to-dark transitions (Goltsev et al. 2009; Strasser et al. 2010; Kalaji et al. 2012).”
Special issue in honour of Prof. Reto J. Strasser - Chlorophyll a fluorescence parameters as indicators of a particular abiotic stress in rice (2020, background): “The reduction in FV/F0 indicates the decrease in the ratio between the rate constants of photochemical and nonphotochemical deactivation of excited Chl molecules (Strasser et al. 2000, 2010; Kalaji et al. 2018).”
Special issue in honour of Prof. Reto J. Strasser - Revisiting JIP-test: An educative review on concepts, assumptions, approximations, definitions and terminology (2020, background): “A limitation can also result from a stress that disconnects PSI from PSII, as found upon dehydration of Haberlea rhodopensis (Strasser et al. 2010).”
Phytotoxic effect of silver nanoparticles in Triticum aestivum: Improper regulation of photosystem I activity as the reason for oxidative damage in the chloroplast (2019, neutral): “Analysis of measured Chl a fluorescence signal was based on a JIP test (Strasser and Strasser 1995, Srivastava et al. 1999, Strasser et al. 2000, 2004, 2010).”